Mechanisms that trigger and sustain a regenerative response following injury
Project available for individuals with self arranged funding.
Efficient repair and replacement of injured or diseased tissues is a major goal in our quest to improve health in an ageing population. The field of regenerative medicine promises to provide a means to tackle many of the most debilitating effects of aging and chronic diseases, by providing a mechanism of replacing aged, injured or diseased tissues with fully functional counterparts. This field has witnessed a great resurgence in recent years, fuelled primarily by advances in stem cell biology. However, the number of findings in regenerative medicine that has resulted in practical clinical applications is fewer than hoped. One reason is that we still have much to learn about the mechanisms that trigger and ultimately promote a healing and regenerative response, following injury. Given the remarkable capacity of amphibians to regenerate fully functional tissues following injury and their ease and tractability as experimental organisms, this project will investigate the mechanisms that trigger and ultimately promote a regenerative response in frog embryos and tadpoles. We have recently discovered a critical role for the production of sustained levels of reactive oxygen species for appendage regeneration in tadpoles (Love et al., 2013). This finding suggests that a low, but sustained, production of ROS triggers and maintains a regenerative response. In this project, the student will establish the molecular mechanism(s) by which ROS facilitates a regenerative response. We predict that findings arising from this PhD project will help pave the way towards the development of novel treatments that will improve the efficiency and speed of tissue repair and regeneration in human patients suffering from acute or chronic wounds and/or diseases.
Love, N.R., Chen, Y., Ishibashi, S., Kritsiligkou, P., Lea, R., Gallop, J.L., Dorey, K. and Amaya, E. (2013) Amputation-induced reactive oxygen species (ROS) are required for successful Xenopus tadpole tail regeneration. Nature Cell Biology, 15:222-228. PMCID: PMC3728553.
Love, N.R., Chen, Y., Bonev, B., Gilchrist, M.J., Fairclough, L., Lea, R., Mohun, T.J., Parades, R., Zeef, L. and Amaya, E. (2011) Genome-wide analysis of gene expression during Xenopus tropicalis tail regeneration. BMC Developmental Biology. 11:70. PMCID: PMC3247858
Love, N.R., Thuret, R., Chen, Y., Ishibashi, S., Sabherwal, N., Paredes, R., Dorey, K., Noble, A.M., Guille, M.J., Sasai, Y., Papalopulu, N. and Amaya, E. (2011) pTransgenesis: A cross-species, modular transgenesis resource. Development. 138:5451-5458. PMCID: PMC3222217.
Ishibashi, S., Cliffe, R. and Amaya, E. (2012) Highly efficient bi-allelic mutations rates using TALENs in Xenopus tropicalis. Biology Open, 1:1273-1276. PMCID: PMC3558749.
Soto, X., Li, J., Lea., R., Dubaissi E, Papalopulu, N. and Amaya E. (2013) Inositol kinase and its product accelerate wound healing by modulating calcium levels, Rho GTPases, and F-actin assembly. Proc. Natl. Acad. Sci. (USA), 110:11029-11034. PMCID: PMC3704016.
Stem Cell Research
This project has a Band 3 fee.
Details of different fee bands are available for UK/EU or International applicants.